Effect of a genetically modified Pseudomonas aureofaciens on indigenous microbial populations of wheat
1994
De Leij, F.A.A.M. | Sutton, E.J. | Whipps, J.M. | Lynch, J.M.
An isolate of Pseudomonas aureofaciens from the phylloplane of sugar beet which was chromosomally modified for monitoring purposes by the insertion of two gene cassettes [km(r)-xylE and lacZY) was introduced to the phytosphere of spring wheat in a number of experiments and the resulting microbial perturbations quantified. Such studies involving innocuous bacterial isolates can serve as a guide in the assessment of risk associated with the release of functionally modified microorganisms. Introductions of P. aureofaciens on seeds caused large microbial perturbations (up to 2 log units) at the seedling stage on seeds and roots. As the inoculated plants matured (tillering, flowering and ripening), perturbations of total microbial populations were found to be non-significant. Microbial perturbation on maturing wheat roots as a result of seed inoculations with P. aureofaciens could only be detected using more sensitive monitoring procedures describing the Pseudomonas community in terms of colony appearance rate on a selective Pseudomonas medium. Spray applications of the marked P. aureofaciens isolate onto the leaf surface of wheat caused no significant perturbations of the indigenous microbial populations present on the phylloplane.
Afficher plus [+] Moins [-]Mots clés AGROVOC
Informations bibliographiques
Cette notice bibliographique a été fournie par National Agricultural Library
Découvrez la collection de ce fournisseur de données dans AGRIS