A PCR-based DNA marker for detection of mutant and normal alleles of the Wx-D1 gene wheat
2001
Shariflou, M.R. | Hassani, M.E. | Sharp, P.J.
To assist waxy wheat breeding a DNA marker was developed to discriminate mutant and normal alleles at the Wx-D1 locus. This polymerase chain reaction-based marker distinguishes the mutant from the normal allele by targeting the previously reported deletion basis of the mutant. The marker codominantly identifies the normal allele of the Wx-D1 gene from the mutant allele originated from the Chinese landrace 'Baihoumai'. However, attempts with a number of primer combinations targeting this deletion failed to amplify the corresponding fragment from an unrelated wheat line (NP150) that has a mutant null allele at the same locus. This indicates that NP150 has a different mutant allele from that of 'Baihoumai'. This marker is a useful tool to identify wheat cultivars with mutant and normal alleles of the Wx-D1 gene, and is used in marker-assisted selection of the Wx-D1 gene in our waxy wheat breeding programme.
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