Superoxide dismutase activity and reduced glutathione levels in Cu-overload liver from sheep
1997
Sansinanea, A.S. | Cerone, S.I. | Streitenberger, S.A. | Garcia, C. | Auza, N.
To explain the cytotoxicity of excessive copper accumulation in the liver of sheep, we measured Cu-Zn superoxide dismutase (SOD) activity, reduced glutathione (GSH) levels and lipid peroxidation in homogenates and subcellular fractions of hepatocytes. Sheep were dosed orally with a 2 per cent solution of CuSO4 on 5 days of the week at the rate of 10 ml per kg body weight to induce the Cu toxicity. Serum copper levels and acid phosphatase (AP) activity were determined at frequent intervals. At time of the first increase of serum AP activity, three treated sheep (group 2A) were euthanased, and at the first day of hemolysis the others treated sheep (group 2B) also were euthanased. We found high liver Cu content and evidence of lipid peroxidation. In whole homogenate, mitochondrial and microsomal fractions, the thiobarbituric acid reacting substances were increased. This was correlated with an increase in the SOD activity and with decrease of the GSH levels. It could be argued that high copper status might have increased the SOD activity and induced lowest levels of GSH. Additionally, lipid peroxidation was induced by Cu-overload.
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