Demonstration of Borna disease virus RNA in peripheral blood mononuclear cells derived from domestic cats in Japan

Nakamura, Y.; Asahi, S.; Nakaya, T.; Bahmani, M.K.; Saitoh, S.; Yasui, K.; Mayama, H.; Hagiwara, K.; Ishihara, C.; Ikuta, K.

Demonstration of Borna disease virus RNA in peripheral blood mononuclear cells derived from domestic cats in Japan

1996

Borna disease virus (BDV) naturally infects horses, sheep, and several other species, including humans, and it is believed to be related to neurological disorders. BDV infection in domestic cats has also been demonstrated by serological assays. We demonstrated for the first time BDV RNA in peripheral blood mononuclear cells from 11 of 83 (13.3%) randomly selected domestic cats in Japan by nested reverse transcriptase-PCR. The BDVs from cats were similar to but slightly different from those from horses and humans, as shown by sequencing the reverse transcriptase-PCR products. None of the cats was positive for both BDV RNA and anti-BDV antibodies.

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Mots clés AGROVOC

animal diseases animal health animals antibodies blood borna disease borna disease virus cats diagnosis genetics horses humans immunology leukocytes methods polymerase chain reaction rna sheep viremia virology

Informations bibliographiques

Publié dans

Journal of clinical microbiology

Volume 34 Numéro 1 Pagination 188 - 191 ISSN 0095-1137

Editeur

Oxford University Press

D'autres materias

Dna primers; Species specificity; Base sequence; Veterinary; Sequence homology; Nucleic acid; Isolation & purification; Amino acid sequence; Viral; Mononuclear; Viral diseases of animals and humans; Cat diseases; Sequence homology; Amino acid; Viral; Molecular sequence data

Langue

anglais

Type

Journal Article; Text

Sur AGRIS depuis: 2024-02-27

Format: MODS

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