Oral administration of beta-1,3/1,6-glucan Macrogard® fails to enhance the mucosal immune response following oral F4 fimbrial immunisation in gnotobiotic pigs
2010
Stuyven, E. | Van den Broeck, W. | Nauwynck, H. | Goddeeris, B.M. | Cox, E.
In this study gnotobiotic animals were used to see if the F4 fimbrial antigen of F4ac+ Escherichia coli is as immunogenic as in conventional pigs. In addition, the adjuvant effect of beta-1,3/1,6-glucans was analysed for the first time in germ-free domestic animals. Oral F4 immunisation of F4 receptor positive (F4R⁺) piglets was able to induce a systemic and mucosal immune response as conventional pigs, although less pronounced. Nevertheless, an IgA response was observed in Peyer's patches and mesenteric lymph nodes. Moreover, a dose dependent effect was observed. Oral administration of beta-glucans for 5 weeks was not able to enhance this F4-specific immune response. The only effect of the beta-glucan treatment we observed was a significantly higher IL-1α mRNA expression in the spleen. No significant changes in total serum antibody concentrations and in the volume of the ileal Peyer's patches were seen.
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