Isolation of a major form of pepsinogen from gastric mucosa of horses

Khittoo, G.; Vermette, L.; Nappert, G.; Lariviere, N.

Isolation of a major form of pepsinogen from gastric mucosa of horses

1991

Khittoo, G. | Vermette, L. | Nappert, G. | Lariviere, N.

In mammalian species studied previously, pepsinogen consisted of biochemically different groups of isozymogens. By use of gel filtration chromatography and electrophoresis, we isolated a predominant pepsinogen from the gastric mucosa of a horse. Peptide mapping with V8 protease revealed differences with its porcine homologue. However, porcine and equine pepsinogens, when activated to pepsin, had a similar pattern of activity when hemoglobin was used as substrate. Those results suggest that differences must exist in the primary structure of the pepsinogens of the 2 species.

Afficher plus [+]

Mots clés AGROVOC

animals chemistry chromatography chromatography electrophoresis enzyme activity gel horses horses ion exchange isolation metabolism pepsin ph proteolysis species differences swine swine

Informations bibliographiques

Publié dans

American journal of veterinary research

Volume 52 Numéro 5 Pagination 713 - 717 ISSN 0002-9645

Editeur

Springer Berlin Heidelberg

D'autres materias

Pepsinogens; Gastric mucosa; Polyacrylamide gel; Interspecific variation; Pepsinogen; Isolation & purification; Endopeptidases; Peptide mapping; Gastric mucosa; Hemoglobins

Langue

anglais

Note

2019-12-04

Type

Text; Journal Article

Sur AGRIS depuis: 2024-02-27

Format: MODS

Fournisseur de données

Cette notice bibliographique a été fournie par National Agricultural Library

Découvrez la collection de ce fournisseur de données dans AGRIS

Consulter Google Scholar