High Responsiveness to Thyroid Hormone of Adult Rat Primary Hepatocytes Cultured on EHS-Gel
1997
Oda, Hiroaki | Nozawa, Katsura | Miyachi, Fuyuko | Shimizu, Ai | Iwasaki, Yukiko | Kakinuma, Atsushi
The induction of malic enzyme gene expression by triiodothyronine and insulin was severely blunted in rat monolayer hepatocytes cultured on type I collagen compared with that in spherical hepatocytes cultured on a reconstituted basement membrane gel (EHS-gel). Although the mRNA level of thyroid hormone receptor β (TRβ) gradually decreased in the monolayer hepatocytes during culture, the mRNA level in the hepatocytes on EHS-gel was maintained at around the in vivo level. Our results suggest that the maintenance of TRβ mRNA on EHS-gel is responsible for the high responsiveness to thyroid hormone in a hepatocyte culture.
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