Genotyping and metabolite characterization of somatic hybrids between Arabidopsis thaliana and Swertia mussotii
2015
Cai, Yunfei | Quan, Taiyong | Yu, Yang | Liu, Guanghua | Xiang, Fengning
Symmetric and polyethylene glycol (PEG)-induced asymmetric fusions between Swertia mussotii and Arabidopsis thaliana protoplasts generated >100 putative hybrid cell lines. Of these, 65 were shown to have a hybrid origin, based on molecular markers, chromosome number, and morphology. An assay directed at the A. thaliana CACTA transposon family detected only three positive lines among the 65 hybrid calli tested. The CACTA sequence amplified from clone D14 was highly homologous with CAC2, indicating that the chromosome content of the hybrid cell lines was largely inherited from S. mussotii. Profiles of secondary metabolites identified a number of S. mussotii- or A. thaliana-specific compounds, as well as significant proportions of compounds not represented in the profile of either parent.
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