Simultaneous saccharification and fermentation of steam exploded duckweed: Improvement of the ethanol yield by increasing yeast titre
2015
Zhao, X. | Moates, G.K. | Elliston, A. | Wilson, D.R. | Coleman, M.J. | Waldron, K.W.
This study investigated the conversion of Lemna minor biomass to bioethanol. The biomass was pre-treated by steam explosion (SE, 210°C, 10min) and then subjected to simultaneous saccharification and fermentation (SSF) using Cellic® CTec 2 (20U or 0.87FPUg−1 substrate) cellulase plus β-glucosidase (2Ug−1 substrate) and a yeast inoculum of 10% (v/v or 8.0×107cellsmL−1). At a substrate concentration of 1% (w/v) an ethanol yield of 80% (w/w, theoretical) was achieved. However at a substrate concentration of 20% (w/v), the ethanol yield was lowered to 18.8% (w/w, theoretical). Yields were considerably improved by increasing the yeast titre in the inoculum or preconditioning the yeast on steam exploded liquor. These approaches enhanced the ethanol yield up to 70% (w/w, theoretical) at a substrate concentration of 20% (w/v) by metabolising fermentation inhibitors.
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