A Gene Homologous to the Streptomyces Chymotrypsin-like Protease (SAM-P20) Gene Is Tandemly Located
1997
Taguchi, Seiichi | Ogawa, Takahiro | Endo, Takeshi | Momose, Haruo
A gene encoding a homolog of the Streptomyces chymotrypsin-like serine protease, SAM-P20, was identified downstream of the sam-p20 gene and designated SAM-P20D. This gene has two tandem Shine–Dalgarno sequences and two initiation codons. We have established vector systems with the function of tyrosinase gene-bone melanin pigmentation as a reporter for sam-p20D gene expression in Streptomyces coelicolor in order to identify the promoter and terminator activities. Using this system, the sam-p20D gene was suggested to be transcribed monocistronically.
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