A new approach for the encapsulation of Saccharomyces cerevisiae using shellac and cellulose nanocrystals
2023
Baek, Jiyoo | Ramasamy, Mohankandhasamy | Cho, David Gunhee | Chung Soo, Colin Chee | Kapar, Sparsh | Lee, Joshua Youngchan | Tam, Kam C.
With the worldwide emergence of health challenges facing humanity in the form of associated immunity and intestinal health, the utilization of effective probiotics is becoming prevalent. We developed a sustainable, biocompatible, rigid, and long-lasting enteric coating system using pH-responsive natural-based materials, shellac (Sh), and cellulose nanocrystals (CNC) which could be utilized as a carrier material for intestinal delivery. As a proof-of-concept, the yeast (Saccharomyces cerevisiae) as a model, was encapsulated in the ShCNC microcapsules with CaCl₂ (Ca). We elucidated the 3D structure of ShCNCCa microcapsules using a confocal laser microscope. A 1–1.3 μm shell consisting of shellac and CNC nanocomplex protected the yeast resulting in high viability and retention of 602.4% under harsh biological conditions. During processing, storage and transport through the gastric environment, the yeasts were protected and then triggered to release in the intestinal environment of pH 7–8. Viscosity synergism and mucoadhesion analysis revealed that the microcapsules bound strongly to the mucus membrane.
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