Production of cloned transgenic cow expressing omega-3 fatty acids
2012
Wu, Xia | Ouyang, Hongsheng | Duan, Biao | Pang, Daxin | Zhang, Li | Yuan, Ding | Xue, Lian | Ni, Daibang | Cheng, Lei | Dong, Shuhua | Wei, Zhuying | Li, Lin | Yu, Ming | Sun, Qing-Yuan | Chen, Da-Yuan | Lai, Liangxue | Dai, Yifan | Li, Guang-Peng
n-3 Polyunsaturated fatty acids (n-3 PUFA) are important for human health. Alternative resources of n-3 PUAFs created by transgenic domestic animals would be an economic approach. In this study, we generated a mfat-1 transgenic cattle expressed a Caenorhabditis elegans gene, mfat-1, encoding an n-3 fatty acid desaturase. Fatty acids analysis of tissue and milk showed that all of the examined n-3 PUAFs were greatly increased and simultaneously the n-6 PUAFs decreased in the transgenic cow. A significantly reduction of n-6/n-3 ratios (P < 0.05) in both tissue and milk were observed.
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