Volatilomic Analyses of Tuscan Juniperus oxycedrus L. and in vitro Cytotoxic Effect of Its Essential Oils on Human Cell Lines
2020
Najar, Basma | Pistelli, Luisa | Buhagiar, Joseph
Chemical composition of different organs and cytotoxic potential of essential oils (EO) of Tuscan Juniperus oxycedrus, was studied in human breast adenocarcinoma (MCF7), human chronic myeloge- nous erythroleukemia (K562) and a human neuroblastoma cell line derived from a highly malignant tumor (SH- SY5Y) cells. GC-MS analyses confirmed the prevalence of monoterpene hydrocarbons (MHs) class in all studied organs with α-pinene as the main compound. The relative abundance of this constituent ranged from 35.3 % in seed EO to exceed 50 % in the mixture (1:1) of leaf and fruit EO. Other MH compounds present in high amounts were myrcene (25.7 % and 10.9 % in seeds and fruits EOs, respectively) and limonene (11.9 % and 7.4 % in leaf and mixture EOs, respectively). Germacrene D, a sesquiterpene hydrocarbon, was the most abundant constituent of fruit EO (30.8 %). Leaf EO showed to be very active on MCF7 (IC50, 19.1 ppm) and K562 (IC50, 35.1ppm) un- derlining the time- and dose-depend way of action. The human neuroblastoma cell line (SH-SY5Y) were almost exclusively sensitive to the fruit EO (IC50, 30.8 ppm).
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