Proteins produced by barley microspores and their derived androgenic structures promote in vitro zygotic maize embryo formation
2003
Paire, A. | Devaux, P. | Lafitte, C. | Dumas, C. | Matthys-Rochon, E.
Maize zygotes formed in planta were isolated and co-cultured with barley microspores. Evidence suggests that culture with microspores and/or conditioned media in barley promoted zygotic embryo formation. In order to characterise active substances present in the conditioned media, we collected medium at various times after the initiation of culture. We showed that proteins appeared over time and that their quantity increased during the course of the culture. Some proteins were glycosylated as revealed by the ConA peroxidase test. The use of the antigen β-glucosyl Yariv reagent has shown that arabinogalactan-proteins (AGPs) were present. In addition, conditioned medium samples were analysed for their oligosaccharide content. New oligosaccharides appear in the course of the culture but they do not seem to affect development. We discuss in details the results in the context of understanding cell-cell interactions between embryo and nurse cells and the possible parallel with that occurs in ovulo between endosperm and embryo.
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