Magnetic Nanoparticles Supported Ionic Liquids Improve Firefly Luciferase Properties
2014
Noori, Ali Reza | Hosseinkhani, Saman | Ghiasi, Parisa | Akbari, Jafar | Heydari, Akbar
Ionic liquids as neoteric solvents, microwave irradiation, and alternative energy source are becoming as a solvent for many enzymatic reactions. We recently showed that the incubation of firefly luciferase from Photinus pyralis with various ionic liquids increased the activity and stability of luciferase. Magnetic nanoparticles supported ionic liquids have been obtained by covalent bonding of ionic liquids-silane on magnetic silica nanoparticles. In the present study, the effects of [γ-Fe₂O₃@SiO₂][BMImCl] and [γ-Fe₂O₃@SiO₂][BMImI] were investigated on the structural properties and function of luciferase using circular dichroism, fluorescence spectroscopy, and bioluminescence assay. Enzyme activity and structural stability increased in the presence of magnetic nanoparticles supported ionic liquids. Furthermore, the effect of ingredients which were used was not considerable on K ₘ value of luciferase for adenosine-5′-triphosphate and also K ₘ value for luciferin.
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