Mechanistic investigations in ultrasound-assisted extraction of astaxanthin from Phaffia rhodozyma MTCC 7536

Batghare, Amit H.; Pati, Saiprasad; Roy, Kuldeep; Moholkar, Vijayanand S.

Mechanistic investigations in ultrasound-assisted extraction of astaxanthin from Phaffia rhodozyma MTCC 7536

2018

Batghare, Amit H. | Pati, Saiprasad | Roy, Kuldeep | Moholkar, Vijayanand S.

This study has dealt with physical mechanism of ultrasound-assisted astaxanthin extraction from dry cell mass (DCM) of Phaffia rhodozyma. Preliminary experiments for screening of extraction solvent revealed highest extraction yield for acetone. Further experiments were performed using mixtures of DMSO and acetone with varying compositions as extraction solvent. The results were analyzed in concurrence with prediction of astaxanthin solubility using UNIFAC (Universal Quasi-Chemical Functional-Group Activity Coefficients) method and simulations of cavitation bubble dynamics. The highest astaxanthin yield of 1536 ± 3 μg/g DCM was obtained for solvent comprising 33% v/v DMSO + 67% v/v acetone. UNIFAC predicted astaxanthin solubility (1.57 g/L) was also highest for this composition, although the convection intensity predicted by bubble dynamics simulations was relatively low. Astaxanthin yield reduced with further increase in DMSO volume fraction in solvent. This result has pointed towards dominant role of solubility of solute rather than intensity of convection in extraction mixture in the extraction yield of astaxanthin.

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