DNA-crowded enzyme complexes with enhanced activities and stabilities
2017
Collins, John | Zhang, Ting | Oh, Sung Won | Maloney, Robert | Fu, Jinglin
We present a robust and simple method to prepare DNA-crowded enzyme complexes by directly assembling long DNA duplexes on the enzyme surface. DNA-crowded enzyme complexes show boosted substrate turnover numbers, and increased stabilities against various storage conditions. They could be potentially scaled up for applications in biomaterials and biotechnology.
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