The efficacy of 2-phenoxyethanol, metomidate, clove oil and MS-222 as anaesthetic agents in the Senegalese sole (Solea senegalensis Kaup 1858)
2009
Weber, R.A. | Peleteiro, J.B. | Martín, L.O García | Aldegunde, M.
The efficacy of four anaesthetic agents (2-phenoxyethanol, metomidate, clove oil and MS-222) was evaluated in the Senegalese sole (Solea senegalensis). It was assumed that stage II of anaesthesia is sufficient to carry out routine aquaculture procedures in less than 3 min, with recovery in less than 5 min. The following optimal doses were determined: 600 mg L⁻¹ of 2-phenoxyethanol (induction 1.50±0.37 and recovery time 1.94±0.56 min), 5 mg L⁻¹ of metomidate (induction 1.50±0.22 and recovery time 3.70±1.18 min), 30 mg L⁻¹ of clove oil (induction 3.16±0.40 and recovery time 3.76±1.01 min) and 75 mg L⁻¹ of MS-222 (induction 2.42±0.20 and recovery time 0.56±0.14 min). The induction times decreased with increasing doses for all of the anaesthetic agents evaluated. Finally, the ability of each anaesthetic agent to prevent a reflex reaction in less than 3 min during simulated blood sampling was evaluated in Senegalese soles of different weights (74±4 g; 213±15 g; 300±12 g), being only achieved in the following cases: 600 mg L⁻¹ of 2-phenoxyethanol and 6 and 8 mg L⁻¹ of metomidate, with fish of 74±4 g, and 600 mg L⁻¹ of 2-phenoxyethanol, 8 mg L⁻¹ of metomidate and 200 mg L⁻¹ of MS-222 with fish of 213±15 g. The most effective of the four anaesthetic agents studied was 2-phenoxiethanol, although all were considered acceptable for use in culture of Senegalese sole.
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