Determination of condensed tannins in complexes with fibre and proteins
1995
Makkar, H.P.S. | Singh, B.
The levels of condensed tannins in tannin-protein complexes dried at 50 degrees C or at 100 degrees C for 2 h, 4 h and 24 h, determined by butanol-HCl reagent, were significantly lower compared to those in freeze-dried or undried complexes. On the other hand, condensed tannins in the fibre fractions (neutral detergent fibre and acid detergent fibre) were statistically similar in samples freeze-dried or dried at 50 degrees C. Significantly lower levels of condensed tannins were observed in fibre fractions dried at 100 degrees C as compared to freeze-dried fibre fractions. The results suggest that for accurate determination of condensed tannins bound to fibre fractions or present as tannin-protein complexes, the fibre fractions or the samples containing tannin-protein complexes should be freeze-dried.
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