Efficient production of recombinant protein in Spodoptera frugiperda/AcNPV system utilizing silkworm hemolymph
1997
Ha, S.H. | Park, T.H.
Silkworm hemolymph when added at 5% (v/v) to medium increased the production of recombinant beta-galactosidase in Spodoptera frugiperda/Autographa californica nuclear polyhedrosis virus (AcNPV) system by 4.5-fold. Silkworm hemolymph also increased the host cell longevity by two-fold. Viability of the host cell was maintained at a constant level for 6 days after baculovirus infection in the medium containing 5% silkworm hemolymph while host cells began to die 3 days after infection in the medium without hemolymph.
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