Development of Immunochromatographic Test System for Detection of Antibiotic Clinafloxacin and Its Application for Honey Control
2021
Zvereva, E. A. | Sotnikov, D. V. | Belichenko, K. A. | Hendrickson, O. D. | Shanin, I. A. | Zherdev, A. V. | Dzantiev, B. B.
To ensure express control of food quality and safety, we developed an immunochromatographic test system for detection of the antibiotic clinafloxacin. The test system implements an indirect competitive immunoassay format based on the interaction of unmodified specific polyclonal antibodies with the antibiotic potentially present in the sample and the antibiotic–protein conjugate immobilized on the working membrane of the test strip. The detection of immune complexes formed on the membrane is carried out using a conjugate of anti-species antibodies and gold nanoparticles. We determined immunochromatography conditions that ensure the achievement of the minimum detection limit. At the assay duration of 20 min, the instrumental and visual detection limits of clinafloxacin comprise 0.3 and 10 ng/mL, respectively. The selectivity of the test system in relation to antibiotics from the class of fluoroquinolones was studied. To ensure the control of the contamination of honey with clinafloxacin and structurally related fluoroquinolones, we identified an optimal sample preparation regimen and confirmed the efficacy of the developed test system.
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