Production and characterization of casein hydrolysates with a high amino acid Fischer's ratio using immobilized proteases
2004
Pedroche, Justo | Yust, Maria M. | Lqari, Hassane | Giron-Calle, Julio | Vioque, Javier | Alaiz, Manuel | Millan, Francisco
A procedure using immobilized enzymes has been developed to obtain protein hydrolysates with a high Fischer's ratio (branched-chain amino acids/aromatic amino acids (AAA)) from bovine casein. Pre-digestion with trypsin was followed by treatment with chymotrypsin, which generated a hydrolysate enriched in peptides with AAA at the carboxyl end. Carboxypeptidase A was then used to remove these AAA. A fraction, which represents 24% of total hydrolyzed proteins, with a Fischer's ratio of 30.6, and Phe+Tyr content below 1.4% of the total, was purified from this hydrolysate by gel filtration in a Biogel P2 column. This material could be used for the treatment of patients suffering from certain hepatic encephalophaties, tyrosinemia and phenylketonuria.
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