Enhanced excretion of extracellular polymeric substances associated with nonylphenol tolerance in Dictyosphaerium sp
2020
Cheng, Qilu | Jiang, Ying | Jin, Zhuo | Hui, Cai | Xu, Ligen | Zhou, Qifa | Zhao, Yuhua | Du, Linna | Jiang, Hui
Dictyosphaerium sp. is tolerant to nonylphenol (NP); however, knowledge regarding the mechanisms involved in NP tolerance is limited. In this study, a batch of algal culture experiments were carried out to elucidate the underlying mechanisms by investigating the production and composition of extracellular polymeric substances (EPS) in algae exposed to NP. The excretion of EPS was significantly enhanced (P < 0.001) in algae exposed to 4 and 8 mg/L of NP. The polysaccharides in soluble EPS and the proteins in bound EPS were specifically overproduced. The three-dimensional excitation and emission matrix fluorescence spectra analyses indicated that tyrosine- and tryptophan-like substances were the main functional compositions in the proteins of EPS. In addition, enhanced EPS secretion significantly alleviated the toxicity of NP to the algae by the reduction of cell internalization, as indicated by the higher IC₅₀, biomass, and cell growth rate in the algae with EPS. These discoveries along with the characterizations by algal cell surface hydrophobicity analysis, scanning electron microscopy, and Fourier transform infrared spectra spectroscopy demonstrated the vital role of EPS in the algal resistance to NP.
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