Determination of selenium balance in healthy children by aas-hydride generation and by inaa technique
2002
Adányi, N. | Váradi, M. | Sziklai-László, I. | Snyder, P. | Snyder, Robert D. | Cser, M. Á
Total daily Se intake was determined by duplicate diet collection, venous blood samples were taken and urine was collected over 24 h in order to measure selenium input and output in healthy, American and Hungarian children aged 8 to 17 living in Budapest. The American children consumed not only locally processed food. Food samples were weighed, mixed, homogenised and the Se content was determined by Instrumental neutron activation analysis (INAA). The Se concentration of blood, plasma and urine samples was determined by atomic absorption spectrometry-hydride generation (AAS-HG) after wet digestion. Se intake calculated for wet weight was 62±18.5 mg/day in American children. In the Hungarian children the mean Se intake was about 35% less than in the Americans. Se concentrations in plasma were 0.84±0.16, in whole blood 1.13±0.17 mmol l<sup>-1</sup> in the Americans, higher than those in healthy Hungarian children (0.64±0.10 and 0.83±0.12 mmol l<sup>-1</sup>, respectively) of similar age and gender. Urinary Se output calculated for creatinin was higher in the children from abroad (27.0±9.5 mg Se/day/g creatinin) compared to Hungarians (11.0±5.0 mg Se/day/g creatinin).
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