The pollution level of the blaOXA-58 carbapenemase gene in coastal water and its host bacteria characteristics
2019
Xin, Rui | Zhang, Kai | Wu, Nan | Zhang, Ying | Niu, Zhiguang
This paper investigated 10 carbapenemase genes and selected the hosts of these genes in the estuary of Bohai Bay. The results showed that the OXA-58 producer accounted for a large percentage of carbapenem resistant bacteria in the sampling points, whereas the VIM, KPC, NDM, IMP, GES, OXA-23, OXA-24, OXA-48 and OXA-51 producers were not detected in the study. In addition, 9 bacterial genera with 100% identical blaOXA₋₅₈ sequences, including Pseudomonas, Rheinheimera, Stenotrophomonas, Shewanella, Raoultella, Vibrio, Pseudoalteromonas, Algoriphagus, Bowmanella and Thalassospira, were isolated from seawater. It is suggested that the host of blaOXA₋₅₈ gene were varied and many kinds of them could survive in the seawater. Moreover, we preformed the quantitative RT-PCR and the result shown the abundance of blaOXA₋₅₈ fluctuated between 2.8×10⁻⁶ copies/16S and 2.46×10⁻⁴ copies/16S, which was of the same order of magnitude as some common antibiotic resistance genes in environment. Furthermore, the variation trend of blaOXA₋₅₈ gene suggested that pollution discharge and horizontal gene transfer could contribute to the increase of the gene in coastal area.
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