Possibility for Application of Extracellular Protease of Micromycete Aspergillus ochraceus for Determining Factor X Content in Human Blood Plasma
2019
Orekhova, A. V. | Osmolovskiy, A. A. | Kreyer, V. G. | Baranova, N. A. | Egorov, N. S.
It has been shown that factor X activity determined in normal plasma using Aspergillus ochraceus protease is comparable with the activity determined with a commercial analogue, a protease from Russell’s viper venom (RVV-X®). It was revealed that the protease of A. ochraceus, along with the RVV-X® preparation, can be used to determine the content of factor X in the plasma with its reduced content. A study of the protease activity of A. ochraceus as an activator to factor X showed that it is slightly higher compared to the snake venom preparation, which can make A. ochraceus protease a promising substitute for the snake activator in diagnostics for determining the content of factor X.
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