Saponins isolated from the leaves of Panax notoginseng protect against alcoholic liver injury via inhibiting ethanol-induced oxidative stress and gut-derived endotoxin-mediated inflammation

Saponins isolated from the leaves of Panax notoginseng protect against alcoholic liver injury via inhibiting ethanol-induced oxidative stress and gut-derived endotoxin-mediated inflammation

2015

Effects of saponins from leaves of Panax notoginseng (LPNS) against alcoholic liver injury were evaluated, and their probable molecular mechanisms were further elucidated in a mouse model of chronic-plus-single-binge ethanol feeding. Alcohol exposure dramatically increased plasma levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triacylglycerol (TG) and endotoxin; these elevations were attenuated by treatment with LPNS at a dose of 100 or 300 mg/kg. LPNS was also found to suppress thiobarbituric acid reactive substances (TBARS) production, enhance glutathione (GSH) level and superoxide dismutase (SOD) activity in liver. The protective of LPNS against oxidative stress was associated with down-regulation of hepatic cytochrome P450 2E1 (CYP2E1), and upregulation of nuclear factor erythroid 2-related factor 2 (Nrf2) and haem oxygenase-1 (HO-1). Additionally, LPNS ameliorated gut leakiness via upregulating the expressions of tight junction proteins, and suppressed endotoxin-mediated inflammation via down-regulating toll-like receptor-4 (TLR-4), CD14 and p-NF-κB p65. These results demonstrate that treatment with LPNS effectively protects against alcoholic liver injury through reducing ethanol-induced oxidative stress and ameliorating gut-derived endotoxin-mediated inflammation.

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Mots clés AGROVOC

animal models ast endotoxins ethanol glutathione gsh hplc inflammation inflammation leaves liver mda oxidative stress oxidative stress saponins sds-page sod superoxide dismutase triacylglycerols

Informations bibliographiques

Publié dans

Journal of functional foods

Volume 19 Pagination 214 - 224 ISSN 1756-4646

Editeur

Elsevier Ireland Ltd

D'autres materias

Alt; Nf-κb; Toll-like receptor 4; Lpns; Lpo; Hrp; Pvdf; Cyp2e1; Ginsenoside rb2 (pubchem cid: 6917976); Ho-1; Tg; Lbp; Ros; Ginsenoside rd (pubchem cid: 24721561); Panax notoginseng; Il-6; Lw; Tbars; Aspartate transaminase; Pmsf; Tight junctions; Ginsenoside rb3 (pubchem cid: 6440398); Ginsenoside rc (pubchem cid: 12855889); Thiobarbituric acid-reactive substances; Gut-derived endotoxin; Alcoholic liver injury; Lps; Cytochrome p-450; Alanine transaminase; Saponins from the leaves of panax notoginseng; Bw; Ald; Tlr4; Zo-1; H2o2; Ginsenoside rb1 (pubchem cid: 9898279); Tnf-α; Nrf2

Langue

anglais

Type

Journal Article; Text

Sur AGRIS depuis: 2024-02-28

Format: MODS

Fournisseur de données

Cette notice bibliographique a été fournie par National Agricultural Library

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Liens

DOI https://dx.doi.org/10.1016/j.jff.2015.09.029

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