NMR studies of a bacterial cell culture medium (LB Broth): cyclic nucleosides in yeast extracts
1990
Rayner, Michael H. | Sadler, Peter J. | Scawen, Michael D.
The composition of LB broth (tryptone, yeast extract and NaCl) was investigated by ¹H, ³¹P‐NMR spectroscopy, FPLC and gel electrophoresis. An enexpected finding was the high level of 2′,3′‐cyclic nucleotides, detected by characteristic ³¹P‐NMR resonances in the region 20–21 ppm, originating from the yeast component. ³¹P‐NMR resonances for cyclic nucleotides were observed during the autolysis of Saccharomyces cerevisiae cells, and in model reactions of RNase with RNA.
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