Botanical micelle and its application for direct electrochemical biosensor
2011
Yun, Miao | Wen, Ying | Dong, Jie | Zhou, Weixun | Zhang, Zongrang | Yang, Haifeng
This paper is concerned about the entrapment of horseradish peroxidase (HRP) within botanical inositol hexakisphosphoric (IP₆) micelles for the preparation of enzyme biosensor. The good affinity of IP₆ micelles with the enzyme provides naturally biocompatible microenvironment for the enzyme immobilization, achieving the direct electron transfer between HRP and electrode surface. The resulting biosensor to H₂O₂ detection exhibits a low detection limit of 0.1μmolL⁻¹ (S/N=3), a quick response time (3s), and a long-term stability. The apparent Michaelis–Menten constant is quite tiny about 0.0016mmolL⁻¹.
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