Extraction, purification and application of thermostable and halostable alkaline protease from Bacillus alveayuensis CAS 5 using marine wastes
2014
Annamalai, Neelamegam | Rajeswari, Mayavan Veeramuthu | Balasubramanian, Thangavel
A marine bacterium Bacillus alveayuensis CAS 5 produced protease when grown at 55°C for 60h in 100ml of basal medium containing 1% SSP (w/v) and purified to 7.77 fold with specific activity of 518.78U/mg. The optimum temperature, pH and NaCl for enzyme activity were 50°C, 9 and 35% respectively. The enzyme was highly stable even at 80°C, pH 12, 35% NaCl and presence of ionic, non-ionic and commercial detergents. The protease was investigated for its application as cleansing additive in blood stain removal. The preset study emphasized that marine wastes can be utilized to generate high value-added products and hidden potential in the production of functional foods.
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