Induction of in vitro cultured masses of shoot primordia of hybrid statice and its cryopreservation by vitrification
1997
Matsumoto, T. | Nako, Y. | Takahashi, C. | Sakai, A.
Bulbous structures consisting of meristematic clumps (designated "shoot primordia") were induced from a meristematic culture of a hybrid statice (Limonium altaica Mill. x L. caspium Mill., cv. Blue Symphonet). The shoot tips were cultured in 25 mL of liquid 1/2 Murashige & Skoog (MS) medium supplemented with 0.44 micromolar BA and 0.054 micromolar NAA and 3% sucrose at pH 5.8 by vertically shaking at 2 rpm on rotating stages (1 m in diameter) at 25 degrees C. One month after inoculation of shoot tips, numerous small globular structures were formed and propagated vegetatively at a high rate following subculture. Segments of shoot primordia had developed into plantlets 2 weeks after transfer to solidified 1/2 MS medium supplemented with with 0.44 micromolar BA and 1% sucrose. Plantlets successfully acclimated and grew into normal plants in a greenhouse. Cold-hardened, precultured small segments of shoot primordia were successfully cryopreserved in liquid N by vitrification. Vitrified and warmed segments plated on solidified 1/2 MS medium produced shoots about 21 d after plating. Cultured masses of shoot primordia appear promising for large-scale production and cryopreservation of annual and biennial statice.
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