Ultrasound-assisted extraction of R-phycoerythrin from Grateloupia turuturu with and without enzyme addition
2015
Le Guillard, Cécile | Dumay, Justine | Donnay-Moreno, Claire | Bruzac, Sandrine | Ragon, Jean-Yves | Fleurence, Joël | Bergé, Jean-Pascal
The aim of this study was to compare two processes for the extraction of R-phycoerythrin (R-PE) from the red seaweed Grateloupia turuturu: ultrasound-assisted extraction (UAE) and ultrasound-assisted enzymatic hydrolysis (UAEH). Process efficiencies were both evaluated by the yield of R-PE extraction and by the level of liquefaction. Experiments were conducted at 40 and 22°C, for 6h, using an enzymatic cocktail and an original ultrasonic flow-through reactor. R-PE appeared very sensitive to temperature, thus 22°C is strongly recommended for its extraction by UAEH or UAE. However, the higher processing temperature (40°C) clearly increased the extraction of water-soluble compounds (up to 91% of liquefaction).These two new processes are thus promising alternatives for the extraction of water-soluble components including R-PE, from wet seaweeds, with extraction yields at least similar to conventional solid–liquid extraction.
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