Environmental and chemical effects on growth, resin and rubber production in guayule tissue cultures
1991
Several environmental and chemical factors affected rubber accumulation in guayule (Parthenium argentatum) tissue cultures. Light increased rubber yields in callus and shoot cultures by 50 and 450% respectively. The Mr of rubber in light-grown callus increased by 60%. Daily periods of incubating callus at 4 degrees increased rubber yield by 70% after five weeks. Increasing sucrose levels up to 7% in the medium stimulated rubber synthesis, but higher levels were inhibitory. Osmotic stress significantly inhibited both rubber production and growth. Rubber production in callus was highest when the content of NO3(-) in culture medium was 30 mol% of total N when compared to NH4(+). Two previously reported stimulators of rubber production in whole plants, acetate and DCPTA, also enhanced the levels of rubber in callus cultures.
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