Fractionation of gliadins from common wheat by cation exchange FPLC
1991
Larre, C. | Popineau, Y. | Loisel, W.
Crude gliadin from common wheat was fractionated by rapid cation exchange chromatography (FPLC) on a mono-S column. Buffers used previously for gliadin fractionation on SP-trisacryl M (a soft cation exchange gel) were used first. It was thus possible to compare gliadin fractionations carried out under very similar conditions by cation exchange FPLC and low pressure chromatography. In both cases the elution times of the components were related to their charges at acid pH. Fractionation can be achieved more rapidly on a mono-S column than on SP-trisacryl M, and the resolution is greater. Mono-S FPLC did not yield pure gliadin components, but each gliadin group was separated into several fractions. The elution times and the resolution are slightly modified by the quantity of protein loaded onto the column, but such variations should not be critical in routine analysis of flour samples extracted under standard conditions. A well-controlled column washing procedure is necessary to ensure reproducible chromatographic profiles that can be used routinely to discriminate between common wheat cultivars. The patterns of 18 genotypes were compared. All were distinguishable from each other. This preliminary investigation showed that cation exchange FPLC may provide an alternative method for wheat cultivar identification.
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