In vitro propagation and assessment of genetic uniformity along with chemical characterization in Hildegardia populifolia (Roxb.) Schott & Endl.: a critically endangered medicinal tree
2020
Upadhyay, Anamica | Shahzad, Anwar | Ahmad, Zishan
Hildegardia populifolia (Roxb.) Schott & Endl. is a critically endangered medicinal tree species which also provide high-quality natural fibers. The present study was focused to develop reliable micropropagation protocol where nodal segment explants were inoculated on Murashige and Skoog medium (MS) and Woody Plant Medium (WPM) augmented with various cytokinins alone or with auxins. WPM was more effective producing up to 10.67 ± 0.88 shoots per explant with average shoot length of 4.20 ± 0.10 cm from nodal explants cultured for 6 wk on medium containing 5.0 μM 6-benzylaminopurine (BA) and 2.0 μM 1-naphthaleneacetic acid (NAA). Microshoots rooted efficiently with a 24-h pulse treatment ex vitro with 200 μM indole-3-butyric acid (IBA) producing 5.33 ± 0.33 roots per microshoot with mean root length of 4.77 ± 0.07 cm. The ex vitro rooting and simultaneous acclimatization resulted in a 98.33 ± 1.67% survival rate in soilrite. Genetic stability was confirmed by using DNA-based molecular markers. Various photosynthetic and biochemical parameter coupled with scanning electron microscopic analysis of leaves revealed the healthy adaptation of the plantlets to natural conditions. Gas chromatography and mass spectrometry analysis of leaf of mother and regenerated plantlets was performed to compare secondary metabolites.
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