In house validation of an HPLC-DAD method for the simultaneous determination of specific bioactive compounds in southern Brazilian propolis
2019
Machado, Christiane Schineider | Finger, Daiane | Felsner, Maria Lurdes | Torres, Yohandra Reyes
The levels of selective bioactive compounds in propolis produced in the state of Paraná, Brazil, were compared with green (São Paulo and Minas Gerais), brown (Santa Catarina), and red (Bahia) propolis. The suitability of the HPLC-DAD method was checked: R² > 99% and no evidence of lack-of-fit (p > 0.01); recoveries within 84.30–119.52% (only 3,4-di-O-caffeoylquinic acid recovery was higher, namely 138.77%); precision up to 11.76% (repeatability) and 14.56% (intermediate); LODs and LOQs of 0.74–3.02 µg/ml and 2.48–10.07 µg/ml, were achieved. p-coumaric and 4-hydroxy-3,5-diprenylcinnamic acids and kaempferide were widely spread in Brazilian propolis. Also, high levels of 3,5-di-caffeoylquinic and 3,4-di-O-caffeoylquinic acids were found in most propolis from Paraná and in green propolis; 4-O-caffeoylquinic acid was abundant in most propolis from Paraná but not in propolis from Minas Gerais, Santa Catarina, and Bahia.
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