Simultaneous Detection of Glutathione and Hydrogen Polysulfides from Different Emission Channels
2017
Chen, Wenqiang | Yue, Xiuxiu | Zhang, Hui | Li, Wenxiu | Zhang, Liangliang | Xiao, Qi | Huang, Chusheng | Sheng, Jiarong | Song, Xiangzhi
Glutathione (GSH) and hydrogen polysulfides (H₂Sₙ) play crucial roles in many physiological processes. To unravel the complicated interrelationship and cellular cross-talk between GSH and H₂Sₙ, the development of single-molecule fluorescent probes that can selectively sense GSH and H₂Sₙ simultaneously from different emission channels is highly desirable. In this report, we have developed the first dual-detection fluorescent probe, ACC-SePh, which responded to GSH with green fluorescence emission, whereas it reacted with H₂Sₙ and emitted blue fluorescence. The probe exhibited excellent selectivity and sensitivity toward GSH and H₂Sₙ over other common reactive sulfur species, such as Cys, Hcy and H₂S. Importantly, we also demonstrated that ACC-SePh can be used for dual-channel imaging of endogenous GSH and H₂Sₙ in living RAW264.7 cells.
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