Comparative Studies on DNA-Binding Mechanisms between Enantiomers of a Polypyridyl Ruthenium(II) Complex
2022
Nie, Yanhong | Dai, Zhongming | Fozia, | Zhao, Guangyao | Jiang, Jianrong | Xu, Xu | Ying, Ming | Wang, Yu | Hu, Zhangli | Xu, Hong
A pair of ruthenium(II) complex enantiomers, Δ- and Λ-[Ru(bpy)₂MBIP]²⁺ (bpy = 2,2′-bipyridine, MBIP = 2-(3-bromophenyl)imidazo[5,6-f]phenanthroline), were designed, synthesized, and characterized. Comparative studies between the enantiomers on their binding behaviors to calf thymus DNA (CT-DNA) were conducted using UV−visible, fluorescence, and circular dichroism spectroscopies, viscosity measurements, isothermal titration calorimetry, a photocleavage experiment, and molecular simulation. The experimental results indicated that both the enantiomers spontaneously bound to CT-DNA through intercalation stabilized by the van der Waals force or the hydrogen bond and driven by enthalpy and that Δ-[Ru(bpy)₂MBIP]²⁺ intercalated into DNA more deeply than Λ-[Ru(bpy)₂MBIP]²⁺ did and exhibited a better DNA photocleavage ability. Molecular simulation further indicated that Δ-[Ru(bpy)₂MBIP]²⁺ more preferentially intercalated between the base pairs of CT-DNA to the major groove, and Λ-[Ru(bpy)₂MBIP]²⁺ more favorably intercalated to the minor groove. These research findings should be very helpful to the understanding of the stereoselectivity mechanism of DNA-bindings of metal complexes, and be useful for the design of novel metal-complex-based antitumor drugs with higher efficacy and lower toxicity.
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