Interaction of aflatoxin G₁ with free DNA in vitro and possibility of its application in removing aflatoxin G₁
2021
Li, Junsheng | Feng, Zhen | Wang, Jingting | Huang, Guoxia | Yan, Liujuan
The present study sought to evaluate the interaction between aflatoxin G₁ and free DNA in vitro through different analytical techniques. The UV–visible spectra results showed that the structure of DNA might be changed with a new aflatoxin G₁-DNA complex forming, which indicated that the interacting mode between them was the intercalating mode. The DNA melting temperature increased by 12.80 °C, suggesting that the DNA double helix structure was more compact and stable through intercalation. The circular dichroism (CD) spectra results indicated that the interaction of aflatoxin G₁ with DNA induced the DNA base stacking changes. The results of agarose gel electrophoresis and fluorescence microscope further verified that the interacting mode between aflatoxin G₁ and DNA was intercalation mode. According to the fluorescence spectrum data, the binding constant was calculated 6.24 × 10⁴ L·mol⁻¹. The thermodynamic results demonstrated that the reaction of aflatoxin G₁ intercalating to DNA was a spontaneous reaction. The elimination results suggested that aflatoxin G₁ could be enriched and removed by DNA intercalation through magnetic beads separation, with the removal efficiency of 93.73%. The study results would provide a theoretical basis for establishing a new aflatoxin removal method based on DNA intercalation.
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