Green analytical methods of metals determination in biosorption studies
2019
Chojnacka, Katarzyna | Mikulewicz, Marcin
The paper reviews state-of-the-art in analytical methods designated for determination of metal ions bound to cellular surfaces in biosorption studies. Although there are 13 750 papers on biosorption (ISI Web of Science), none review analytical methods used to characterize this method. Biosorption is the equilibrium process, whereby metal ions are bound to functional groups (carboxyl, sulfhydryl, amino, phosphoryl), which are the components of the cell wall. Different methods are used to study biosorption: that measure the total content of the sorbate bound by the sorbent, such as ICP-OES/MS are highly sensitive and precise, but are not green analytical methods. Main techniques considered as green are semi-quantitative methods that require revalidation and standardization for this application. Specific analytical methods show different picture of the analyte: total content in the biomass, surface density, mechanism identification. The paper discusses how analytical techniques are used in combination to explore the process of biosorption.
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