Apoptotic activity of ethanol extract from Styrax Japonica Siebold et al Zuccarini in HepG2 cells
2010
Lee, Jin | Lim, Kye-Taek
Ethnopharmacological relevance: Styrax japonica Siebold et al Zuccarini (SJSZ) has been used to heal inflammation and bronchitis as an herbal plant in Korea. Aims of the study: The purpose of the present study is to determine whether the ethanol (EtOH) extract of SJSZ induces the programmed cell death (apoptosis) in human hepatoma HepG2 cells. Materials and methods: It was evaluated cytotoxicity using MTT assay, amount of intracellular reactive oxygen species (iROS) and Ca²⁺ using fluorescence. Activities of apoptotic relevant factors [Bid, cytochrome c, caspase-9, -3, and poly-(ADP-ribose) polymerase (PARP)] were measured by Western blot. Results: The results in this study indicated that ethanol extract of SJSZ (75μg/ml) stimulates to increase amount of iROS, Ca²⁺, and the apoptotic relevant factors [Bid, cytochrome c, caspase-9, -3, and poly-(ADP-ribose) polymerase (PARP) in the HepG2 cells. Conclusion: The results in this study indicated that ethanol extract of SJSZ (75μg/ml) induces programmed cell death (apoptosis) in the HepG2 cells. Therefore, we speculate that ethanol extract of SJSZ could be used for healing of hepatocarcinoma as one of chemotherapeutic agents.
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