L- Lactic acid fermentation by Enterococcus faecium: a new isolate from bovine rumen
2015
Sun, Wentao | Liu, Jianjun | Xu, Hui | Li, Wenjing | Zhang, Jing
OBJECTIVES: To obtain a novel adaptable stain that can produce enantioselective L-lactic acid efficiently, reduce the operational cost of fermentation process and be suitable for scale production. RESULTS: Enterococcus faecium S.156 produced 126 g L-lactic acid/l with high optical purity (99.7 %), high productivity (5.25 g/l.h) and a conversion ratio >90 %. L-lactic acid production remained steady from 32 to 40 °C and at pH values from 5.5 to 6.5. O₂ made no difference to both biomass growth and the L-lactic acid fermentation. 8 mg folic acid/l combined with 2 mM Fe²⁺ substituted for 6 g yeast extract/l, which is a cost-saving. CONCLUSIONS: The special characteristics and economic traits of E. faecium S.156 make it suitable for industrial-scale production of L-lactic acid.
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