Inhibition of pig oocyte in vitro fertilization by the action of components of the zona pellucida
1994
Fierro, R. | Bonilla, E. | Casas, E. | Jimenez, I. | Ducolomb, Y. | Betancourt, M.
The aim of the present study was to determine whether the previous addition of porcine zona pellucida (ZP) components to spermatozoa of the same species has an inhibitory effect on in vitro fertilization (IVF). Boar spermatozoa were exposed to whole porcine solubilized zona pellucida (SZP), ZP glycoproteins (55 kDa and 90 kDa) and peptides (37 kDa, 40 kDa and 68 kDa). Doses tested were 40, 70 and 100 micrograms/ml. In vitro fertilization was clearly inhibited by each component when the oocytes were compared with those fertilized with untreated spermatozoa. All the components had an effect in a dose dependent manner.
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