Isolation and characterization of an extracellular glucan produced by Leuconostoc garlicum PR
2011
Capek, P. | Hlavoňová, E. | Matulová, M. | Mislovicová, D. | Růžička, J. | Koutný, M. | Keprdová, L.
White and highly viscous extracellular polysaccharide composed of d-glucose residues has been isolated from culture medium of Leuconostoc garlicum PR by acetone precipitation followed by dialysis and freeze-drying. The crude biopolymer was recovered in the yield of 50g/L of rich culture medium and showed an apparent molecular mass over 2×10⁷. Chemical and spectroscopic studies revealed almost linear character of the bacterial biopolymer composed of α-1,6-linked d-glucopyranosyl backbone carrying a low content of branched points at C-2, C-3 and C-4. Moreover, a low content (∼2%) of α-1,4-linked d-glucopyranosyl residues has been determined in the extracellular biopolymer.
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