Effect of ABA, arginine and sucrose on protein content of date palm somatic embryos
2009
Sghaier, Besma | Kriaa, Walid | Bahloul, Mouna | Jorrin-Novo, Jesus V. | Drira, Noureddine
Abscisic acid (ABA), arginine and sucrose were evaluated for their effects on the morphology, germination rates and protein content of date palm somatic embryos (SE). Different concentrations of these supplements in the culture medium were used. The comparative study of SE length and thickness between treated and untreated SE revealed no differences, except for ABA (20μM), which increased thickness. A decrease of water content (WC) in favor of an increase in dry weight (DW) was observed in all treated SE, especially with sucrose (90gl⁻¹) and ABA (20μM). Only ABA (20 and 4μM) caused a proliferation rate of the cultures higher than those in the control. Although all the tested compounds increased protein content, ABA (20μM) was more effective in protein enrichment than arginine and sucrose treatments. The SDS-PAGE protein profiles showed a significant difference between treated and untreated SE. A protein band of 22kDa, identified as glutelin in a previous work, was accumulated after treatment with 20μM ABA or 3mM arginine. These findings may contribute to further understanding of the mechanisms involved in the accumulation of specific storage proteins in several plants.
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