Production and Characterization of Fungal Lignin Peroxidase and Its Role in Polyvinyl Chloride Degradation
2017
Khan, Imran | Sajjad, Wasim | Zada, Sahib | Sajjad, Wasim | ʻAlī, Barkat | Huq, Abdul
With the passage of time, durability and stability of plastic has been upgraded continuously and now this material is resistant to several environmental effects. The present study aim was to study the Phanerochaete chrysosporium ability of lignin peroxidase enzyme production and its potential of polyvinyl chloride degradation. The fungal lignin peroxidase was qualitatively confirmed by using methylene blue assay. Veratryl alcohol was used for quantitative analysis of lignin peroxidase at 25°C. Effect of various factors such as temperature, pH, incubation time and glucose concentration on enzyme production was studied in triplicate. Purified enzyme molecular weight was determined through sodium dodecyl sulfate gel. Degradation of pure polyvinyl chloride was investigated in crude lignin peroxidase mixture in shaking incubator at 30°C. The fungal isolate efficiently produced lignin peroxidase in mineral salt medium having 1% glucose as a carbon source. Maximum enzyme activity of 0.479 U/mL was observed at pH 7.0, and 0.482 U/mL at temperature 30°C, while at 1% glucose concentration 0.473 U/mL activity was recorded. The purified enzyme molecular weight was 37 kDa. The crude enzyme mixture considerably caused corrosion on polyvinyl chloride surface, which was qualitatively confirmed with scanning electron microscopy. The result obtained from Fourier Transform Infra-Red (Jasco FT/ IR – 620) also exhibited the changes occurred in the functional groups after treatment with crude enzyme. As plastic is the main source of environmental destruction, therefore, this biodegradation of plastic with fungal enzymes may play a vital role to overcome this issue.
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