A high-throughput screening method based on the Mizoroki-Heck reaction for isolating itaconic acid-producing fungi from soils
2019
Sano, Mei | Kuroda, Hikari | Ohara, Hitomi | Andō, Hiroshi | Matsumoto, Keiji | Aso, Yuji
In this study, we report a novel method based on the Mizoroki-Heck reaction followed by an iodine test for the screening of itaconic acid-producing fungi from soils. This method is simple, rapid, and requires 10 μL of culture; results are obtained within 1.5 h. The detection limit of itaconic acid in the cultures was 0.13 mM. This is the first report on the direct screening of itaconic acid-producing fungi using a coupling reaction.
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