Genetic Diversity of Chinese and CIP Potato (Solanum tuberosum L.) Germplasm Assessed by Amplified Fragment Length Polymorphism (AFLP) Markers
2013
Wang, Fang | Li, Fang-di | Wang, Jian
The genetic diversity of 123 potato clones, consisting of 103 clonal accessions from the International Potato Center (CIP) and 20 Chinese cultivars, was assessed using amplified fragment length polymorphism (AFLP) markers. Ten polymorphic primer combinations were selected to analyse and compare the diversity of the two sources of clones. A total of 521 reproducible bands were amplified, of which 488 were polymorphic. These AFLP markers were analysed to estimate the genetic distance (GD) and genetic similarity (GS) coefficient of all clones. The GD between pairs of clones ranged from 0.03 to 0.48. Nei’s gene diversity index was between 0.236 and 0.387, with an average of 0.330. The index of Shannon’s information varied from 0.361 to 0.567 with an average of 0.498. A high degree of polymorphism was observed with an average of 93.6% loci found to be polymorphic. Cluster analysis showed that the CIP accessions were grouped together at the GS 0.59 clustering line, whereas most Chinese cultivars grouped at the GS 0.82 clustering line. The diversity in CIP potato resources was found to be higher than that of the Chinese cultivars, indicating that the genetic base of Chinese potato cultivars is narrow and may benefit from broadening.
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