Effect of thiolactomycin of fatty acid synthesis in peas
1995
Jones, Al | Dancer, J.E. | Harwood, J.L.
Thiolactomycin inhibited fatty acid synthesis in isolated pea chloroplasts. Using [1-14C]acetate as precursor, an I50 value of ca 20 micromolar was obtained for a (R, S) mixture of thiolactomycin. The antibiotic inhibited the ketoacyl-ACP synthase (condensing enzyme: KAS) and the acetyl-CoA: ACP (ACAT) reactions. An examination the relative inhibition of the three condensing enzymes revealed that their order of sensitivity to thiolactomycin was KAS II > KAS I > KAS III.
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