Blue-green fluorescence excited by UV laser on leaves of different species originates from cutin and is sensitive to leaf temperature
1994
Bongi, G. | Palliotti, A. | Rocchi, P. | Moya, I. | Goulas, Y.
Under ultra-violet excitation, intact leaves generate a strong blue-green fluorescence emission with several bands. Their integrated energy is 6 to 11 times the energy released by chlorophyll a bands (Chappelle et aL 1984, Applied Optics 23, 134-138). This paper provides evidence that the blue-green fluorescence emission comes mainly from outer epidermal layers of the leaves and can be transferred on a quartz lamina by quickly dipping the leaves in organic solvents with subsequent solvent evaporation. Blue-green fluorescence displays a diffusion-controlled quenching of fluorescence intensity between 4 degrees C (high fluorescence) and 37 degrees C (low fluorescence). The blue-green fluorescence emissivity is not linked to short-term metabolic effects other than leaf temperature, but epidermis adaptations both to drought and to excessive radiation increase emissivity.
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